Coding

Part:BBa_K4790055:Design

Designed by: Yi Zheng   Group: iGEM23_NNU-CHINA   (2023-08-14)


ASR1(node 59)-M17A


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 799
    Illegal NheI site found at 721
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 799
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 799
    Illegal PstI site found at 100
    Illegal PstI site found at 232
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We should select direction of mutation which is predicted the highest possibility. Mutation should refer to Codon preference in E.coli.


Source

It does not come from any genomic sequence. Node 59 comes from Ancestral Sequence Reconstruction (ASR). The mutation site is predicted by machine learning.

References